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Apoptosis,, or programmed cell death, is an essential process by which multi-cellular organisms ensure that critically damaged or mutated cells are removed. The process is regulated by a complex interplay of proteins and, until reaching “the point of no return”, can be halted or reversed at any point.

The ,kit, allows multi-color labeling in conjunction with experiment-specific antibodies. DNA fragmentation is an irreversible, committed step in ,apoptosis,, and the labeling of 3’ ends provides an easy measure of cells undergoing ,apoptosis,. Cells may also be analyzed for DNA content using the included propidium iodide/RNase A solution.

Apoptosis,, or programmed cell death, is an essential process by which multi-cellular organisms ensure that critically damaged or mutated cells are removed. The process is regulated by a complex interplay of proteins and, until reaching “the point of no return”, can be halted or reversed at any point.

During ,apoptosis,, DNA is fragmented following the apoptotic activation of intracellular endonucleases. The In Situ ,Apoptosis Detection Kit, is designed to detect fragmented DNA histochemically by TUNEL (TdT-mediated dUTP Nick End Labeling). Via this method, fluorescein-labeled nucleotides are incorporated in situ onto the 3' ends of DNA fragments, allowing histologic localization and ,detection, ...

During ,apoptosis,, DNA is fragmented following the apoptotic activation of intracellular endonucleases. The In Situ ,Apoptosis Detection Kit, is designed to detect fragmented DNA histochemically by TUNEL (TdT-mediated dUTP Nick End Labeling). Via this method, fluorescein-labeled nucleotides are incorporated in situ onto the 3' ends of DNA fragments, allowing histologic localization and ,detection, ...

We used the Annexin V-FITC ,apoptosis detection kit, to determine minor levels of cell death (using both the Annexin V-FITC and PI) in mouse cortical collecting duct cellss (mCCDs). Using this ,kit,, we were able to detect cell death in less than 5% of all cells using fluorescent microscopy.

The ,kit, allows multi-color labeling in conjunction with experiment-specific antibodies. DNA fragmentation is an irreversible, committed step in ,apoptosis,, and the labeling of 3’ ends provides an easy measure of cells undergoing ,apoptosis,. Cells may also be analyzed for DNA content using the included propidium iodide/RNase A solution.

The Annexin V ,Apoptosis Detection Kits, include an Annexin V conjugate or a tagged Annexin V, respectively, as well as binding buffer and partially also propidium iodide (PI) for differentiating between early apoptotic and necrotic cells. The ,kits, allow a one-step staining procedure that takes only 10 …

Detection, of apoptotic cells in mouse tongue tissue using TUNEL Andy Fluor™ 488 ,Apoptosis Detection Kit,. To order. Buy Cat.No. Product Name Ex/Em Unit Price A050 TUNEL Andy Fluor™ 488 ,Apoptosis Detection Kit, 495/520 50 assay $380 Documents. Protocol (PDF): A050 MSDS (PDF): Component A Component C Component E Component G ...

During late-stage ,apoptosis,, loss of cell membrane integrity allows Annexin V binding to cytosolic PS, as well as cell uptake of PI and 7-AAD. Annexin V staining, paired with 7-AAD or PI is widely used to identify apoptotic stages by flow cytometry.

The CF® dye TUNEL Assay ,Apoptosis Detection Kit, label apoptotic cells in fixed tissue sections or cellular samples for fluorescence microscopy or flow cytometry. Product Description. These ,kits, use enzymatic labeling of DNA double-strand breaks to fluorescently stain apoptotic cells in …

Detection, of apoptotic cells in mouse tongue tissue using TUNEL Andy Fluor™ 488 ,Apoptosis Detection Kit,. To order. Buy Cat.No. Product Name Ex/Em Unit Price A050 TUNEL Andy Fluor™ 488 ,Apoptosis Detection Kit, 495/520 50 assay $380 Documents. Protocol (PDF): A050 MSDS (PDF): Component A Component C Component E Component G ...

Kit, size- Convenient sizes (25 and100 assays) Applications- Disruption of mitochondrial transmembrane potential is one of the earliest intracellular events that occur upon induction of ,apoptosis,. The MitoCapture Mitochondrial ,Apoptosis Detection Kit, provides a simple and sensitive in vitro assay for detecting the mitochondrial changes in ,apoptosis,.

Why Use TumorTACS In Situ ,Apoptosis Detection Kit,? The labeling of tumor-containing specimens can be problematic due to the high levels of necrosis in these tissues. During necrosis, cessation of homeostasis, cell swelling, and nuclear membrane rupture generates highly degraded DNA that can interfere with interpretation of data.

During late-stage ,apoptosis,, loss of cell membrane integrity allows Annexin V binding to cytosolic PS, as well as cell uptake of PI and 7-AAD. Annexin V staining, paired with 7-AAD or PI is widely used to identify apoptotic stages by flow cytometry.

In early-stage ,apoptosis,, the plasma membrane excludes viability dyes such as propidium iodide (PI), 7-AAD, or Fixable Viability Dyes such as eFluor™ 660 or eFluor™ 780. These cells will stain with Annexin V but not a viability dye, thus distinguishing cells in early ,apoptosis,.

Kit, size- Convenient sizes (25 and100 assays) Applications- Disruption of mitochondrial transmembrane potential is one of the earliest intracellular events that occur upon induction of ,apoptosis,. The MitoCapture Mitochondrial ,Apoptosis Detection Kit, provides a simple and sensitive in vitro assay for detecting the mitochondrial changes in ,apoptosis,.

The CF® dye TUNEL Assay ,Apoptosis Detection Kit, label apoptotic cells in fixed tissue sections or cellular samples for fluorescence microscopy or flow cytometry. Product Description. These ,kits, use enzymatic labeling of DNA double-strand breaks to fluorescently stain apoptotic cells in …

The Annexin V ,Apoptosis Detection Kits, include an Annexin V conjugate or a tagged Annexin V, respectively, as well as binding buffer and partially also propidium iodide (PI) for differentiating between early apoptotic and necrotic cells. The ,kits, allow a one-step staining procedure that takes only 10 …

Why Use TumorTACS In Situ ,Apoptosis Detection Kit,? The labeling of tumor-containing specimens can be problematic due to the high levels of necrosis in these tissues. During necrosis, cessation of homeostasis, cell swelling, and nuclear membrane rupture generates highly degraded DNA that can interfere with interpretation of data.